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NDUFS6 Antibody

CAB3985
Assay Genie
TargetNDUFS6
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NDUFS6 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4726
  • Target name
    NDUFS6
  • Target description
    NADH:ubiquinone oxidoreductase subunit S6
  • Target synonyms
    CI-13kA, CI-13kD-A, CI13KDA, MC1DN9, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase), NADH-ubiquinone oxidoreductase 13 kDa-A subunit, NADH:ubiquinone oxidoreductase NDUFS6 subunit, complex I 13kDa subunit A, complex I, mitochondrial respiratory chain, 13-kD subunit
  • Host
    Rabbit
  • Protein IDO75380
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS6 antigen affinity purified polyclonal antibody (Catalog # A09082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS6 at approximately 14 kDa. The expected band size for NDUFS6 is at 14 kDa.
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Lane 1: Hela cell lysates; Lane 2: U937 cell lysates probed with NDUFS6 Polyclonal Antibody, Unconjugated (bs-19092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Western blot All lanes: NDUFS6 antibody at 1.1microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
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