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Western Blot Positive WB detected in: Rat brain tissue All lanes: NDUFS8 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa
Western Blot Positive WB detected in: Rat brain tissue All lanes: NDUFS8 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa
Western Blot Positive WB detected in: Rat brain tissue All lanes: NDUFS8 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa

NDUFS8 Antibody

CSB-PA015667LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNDUFS8
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFS8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4728
  • Target name
    NDUFS8
  • Target description
    NADH:ubiquinone oxidoreductase core subunit S8
  • Target synonyms
    CI-23k; CI23KD; complex I 23kDa subunit; complex I-23kD; MC1DN2; NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; NADH-ubiquinone oxidoreductase 23 kDa subunit; TYKY
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00217
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Scientific Description
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (Catalog # A08275-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.
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