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NDUFS8 antibody

GTX114119
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFS8
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFS8 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.35 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4728
  • Target name
    NDUFS8
  • Target description
    NADH:ubiquinone oxidoreductase core subunit S8
  • Target synonyms
    CI-23k; CI23KD; complex I 23kDa subunit; complex I-23kD; MC1DN2; NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; NADH-ubiquinone oxidoreductase 23 kDa subunit; TYKY
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00217
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Scientific Description
    This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy. Ishiyama A et al., 2017 Oct, Neurol Genet
    Read more
  • Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinsons disease and Parkinsons disease with dementia. Garcia-Esparcia P et al., 2018 Jan, Brain Pathol
    Read more
  • Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly. Lim SC et al., 2016 Jun, FASEB J
    Read more

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Figure 1. Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (Catalog # A08275-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.
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