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Western Blot analysis of HT29 cells using APBA2BP Polyclonal Antibody
Western Blot analysis of HT29 cells using APBA2BP Polyclonal Antibody
Western Blot analysis of HT29 cells using APBA2BP Polyclonal Antibody

NECAB3 Antibody

CSB-PA000896
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNECAB3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NECAB3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63941
  • Target name
    NECAB3
  • Target description
    N-terminal EF-hand calcium binding protein 3
  • Target synonyms
    amyloid beta (A4) precursor protein-binding, family A, member 2 binding protein; amyloid beta A4 protein-binding family A member 2-binding protein; APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; EF-hand calcium binding protein 3; Nek2-interacting protein 1; neuronal calcium-binding protein 3; neuronal calcium-binding protein NECAB3; NIP1; N-terminal EF-hand calcium-binding protein 3; STIP3; synaptotagmin interacting protein 2; synaptotagmin interacting protein STIP3; SYTIP2; X11L-binding protein 51; XB51
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96P71
  • Protein Name
    N-terminal EF-hand calcium-binding protein 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NECAB3 using anti-NECAB3 antibody (A11525-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB3 antigen affinity purified polyclonal antibody (Catalog # A11525-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB3 at approximately 44 kDa. The expected band size for NECAB3 is at 44 kDa.
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