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NECAB3 Antibody

CAB15500
Assay Genie
TargetNECAB3
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NECAB3 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID63941
  • Target name
    NECAB3
  • Target description
    N-terminal EF-hand calcium binding protein 3
  • Target synonyms
    amyloid beta (A4) precursor protein-binding, family A, member 2 binding protein; amyloid beta A4 protein-binding family A member 2-binding protein; APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; EF-hand calcium binding protein 3; Nek2-interacting protein 1; neuronal calcium-binding protein 3; neuronal calcium-binding protein NECAB3; NIP1; N-terminal EF-hand calcium-binding protein 3; STIP3; synaptotagmin interacting protein 2; synaptotagmin interacting protein STIP3; SYTIP2; X11L-binding protein 51; XB51
  • Host
    Rabbit
  • Protein IDQ96P71
  • Protein Name
    N-terminal EF-hand calcium-binding protein 3
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NECAB3 using anti-NECAB3 antibody (A11525-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB3 antigen affinity purified polyclonal antibody (Catalog # A11525-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB3 at approximately 44 kDa. The expected band size for NECAB3 is at 44 kDa.
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