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Nectin 2/CD112 Rabbit mAb

A9622
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNECTIN2
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Overview

  • Supplier
    ABclonal
  • Product Name
    Nectin 2/CD112 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5819
  • Target name
    NECTIN2
  • Target description
    nectin cell adhesion molecule 2
  • Target synonyms
    CD112, HVEB, PRR2, PVRL2, PVRR2, nectin-2, herpesvirus entry protein B, poliovirus receptor-like 2, poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92692
  • Protein Name
    Nectin-2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 2/NECTIN2 antigen affinity purified polyclonal antibody (Catalog # A08081-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 2/NECTIN2 at approximately 70-80KD. The expected band size for Nectin 2/NECTIN2 is at 58KD.
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Human U937 cells probed with CD112 Polyclonal Antibody, Unconjugated (bs-2679R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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