Western blot using GeneTexs affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. Primary antibody was used at a 1:350 dilution followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG.
Western blot using GeneTexs affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. Primary antibody was used at a 1:350 dilution followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG.
NEDD4 antibody
GTX48706
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNEDD4
Overview
- SupplierGeneTex
- Product NameNEDD4 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:300-1:500. ELISA: 1:20000-1:35000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.64 mg/ml
- ConjugateUnconjugated
- Gene ID4734
- Target nameNEDD4
- Target descriptionNEDD4 E3 ubiquitin protein ligase
- Target synonymscell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD4-1; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; receptor-potentiating factor 1; RPF1
- HostRabbit
- IsotypeIgG
- Protein IDP46934
- Protein NameE3 ubiquitin-protein ligase NEDD4
- Scientific DescriptionNEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddles Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203