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Western Blot analysis of Raji cell using NEDD9 Polyclonal Antibody at dilution of 1:650
Western Blot analysis of Raji cell using NEDD9 Polyclonal Antibody at dilution of 1:650
Western Blot analysis of Raji cell using NEDD9 Polyclonal Antibody at dilution of 1:650

NEDD9 Polyclonal Antibody

E-AB-15091
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NEDD9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
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