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NEI3 / NEIL3 Antibody (460-540 aa, C-terminal)

LS-C384809
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNEIL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NEI3 / NEIL3 Antibody (460-540 aa, C-terminal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:40000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55247
  • Target name
    NEIL3
  • Target description
    nei like DNA glycosylase 3
  • Target synonyms
    FGP2, FPG2, NEI3, ZGRF3, hFPG2, hNEI3, endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase hFPG2, DNA glycosylase/AP lyase Neil3, endonuclease VIII-like 3, nei endonuclease VIII-like 3, nei-like protein 3, zinc finger, GRF-type containing 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEIL3 antigen affinity purified polyclonal antibody (Catalog # A05497-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEIL3 at approximately 70 kDa. The expected band size for NEIL3 is at 62-68 kDa.
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