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Western Blot analysis of Hela cell using NEIL3 Polyclonal Antibody
Western Blot analysis of Hela cell using NEIL3 Polyclonal Antibody
Western Blot analysis of Hela cell using NEIL3 Polyclonal Antibody

NEIL3 Antibody

CSB-PA003396
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNEIL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEIL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55247
  • Target name
    NEIL3
  • Target description
    nei like DNA glycosylase 3
  • Target synonyms
    FGP2, FPG2, NEI3, ZGRF3, hFPG2, hNEI3, endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase hFPG2, DNA glycosylase/AP lyase Neil3, endonuclease VIII-like 3, nei endonuclease VIII-like 3, nei-like protein 3, zinc finger, GRF-type containing 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAT5
  • Protein Name
    Endonuclease 8-like 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEIL3 antigen affinity purified polyclonal antibody (Catalog # A05497-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEIL3 at approximately 70 kDa. The expected band size for NEIL3 is at 62-68 kDa.
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