
Detection of human NEK2 protein using GeneTex NEK2 1B6 monoclonal antibody (GTX70281) in Jurkat whole cell extract.
NEK2 antibody [1B6]
GTX70281
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNEK2
Overview
- SupplierGeneTex
- Product NameNEK2 antibody [1B6]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1B6
- Concentration0.43 mg/ml
- ConjugateUnconjugated
- Gene ID4751
- Target nameNEK2
- Target descriptionNIMA related kinase 2
- Target synonymsHsPK21, NEK2A, NLK1, PPP1R111, RP67, serine/threonine-protein kinase Nek2, NIMA (never in mitosis gene a)-related kinase 2, nimA-like protein kinase 1, nimA-related protein kinase 2, protein phosphatase 1, regulatory subunit 111
- HostMouse
- IsotypeIgG1
- Protein IDP51955
- Protein NameSerine/threonine-protein kinase Nek2
- Scientific DescriptionThis gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (GTX70281) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (GTX70281) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX70281/GTX70281_44223_20210212_WB_w_23061221_976.webp)






![Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (GTX638872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638872/GTX638872_T-45117_20230811_WB_treatment_MG132_23081619_451.webp)