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Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody

NEMF Antibody

CSB-PA003494
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNEMF
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEMF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9147
  • Target name
    NEMF
  • Target description
    nuclear export mediator factor
  • Target synonyms
    antigen NY-CO-1; caliban homolog; IDDSAPN; nuclear export mediator factor NEMF; NY-CO-1; SDCCAG1; serologically defined colon cancer antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60524
  • Protein Name
    Nuclear export mediator factor NEMF
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SDCCAG1/NEMF using anti-SDCCAG1/NEMF antibody (A09039-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCCAG1/NEMF antigen affinity purified polyclonal antibody (Catalog # A09039-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCCAG1/NEMF at approximately 140 kDa. The expected band size for SDCCAG1/NEMF is at 123,130-140 kDa.
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WB analysis of HeLa and Jurkat cell lysates using GTX87615 NEMF antibody. The lane on the right is blocked with the synthesized peptide.
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