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WB analysis of HeLa and Jurkat cell lysates using GTX87615 NEMF antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HeLa and Jurkat cell lysates using GTX87615 NEMF antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HeLa and Jurkat cell lysates using GTX87615 NEMF antibody. The lane on the right is blocked with the synthesized peptide.

NEMF antibody

GTX87615
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNEMF
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Overview

  • Supplier
    GeneTex
  • Product Name
    NEMF antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9147
  • Target name
    NEMF
  • Target description
    nuclear export mediator factor
  • Target synonyms
    antigen NY-CO-1; caliban homolog; IDDSAPN; nuclear export mediator factor NEMF; NY-CO-1; SDCCAG1; serologically defined colon cancer antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60524
  • Protein Name
    Nuclear export mediator factor NEMF
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SDCCAG1/NEMF using anti-SDCCAG1/NEMF antibody (A09039-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCCAG1/NEMF antigen affinity purified polyclonal antibody (Catalog # A09039-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCCAG1/NEMF at approximately 140 kDa. The expected band size for SDCCAG1/NEMF is at 123,130-140 kDa.
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