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NETO1 Antibody

LS-C674230
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNETO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NETO1 Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81832
  • Target name
    NETO1
  • Target description
    neuropilin and tolloid like 1
  • Target synonyms
    BCTL1, BTCL1, neuropilin and tolloid-like protein 1, brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, neuropilin (NRP) and tolloid (TLL)-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NETO1 using anti-NETO1 antibody (A08303-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse RM-1 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO1 antigen affinity purified polyclonal antibody (Catalog # A08303-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO1 at approximately 70 kDa. The expected band size for NETO1 is at 60 kDa.
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