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ProductsBack/NETO2 Antibody - N-terminal region (OAAB09633)

NETO2 Antibody - N-terminal region (OAAB09633)

OAAB09633
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNETO2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    NETO2 Antibody - N-terminal region (OAAB09633)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID81831
  • Target name
    NETO2
  • Target description
    neuropilin and tolloid like 2
  • Target synonyms
    BTCL2, NEOT2, neuropilin and tolloid-like protein 2, brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, neuropilin (NRP) and tolloid (TLL)-like 2
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NETO2 using anti-NETO2 antibody (A09555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO2 antigen affinity purified polyclonal antibody (Catalog # A09555-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO2 at approximately 65 kDa. The expected band size for NETO2 is at 59 kDa.
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