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Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Neuropilin-1 Recombinant Antibody

BSM-52479R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNRP1
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Overview

  • Supplier
    Bioss
  • Product Name
    Neuropilin-1 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:50-200), IF(IF=1:100-500), Flow-Cyt(Flow-Cyt=1:50), ICC/IF(ICC/IF=1:50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8829
  • Target name
    NRP1
  • Target description
    neuropilin 1
  • Target synonyms
    BDCA4, CD304, NP1, NRP, VEGF165R, neuropilin-1, transmembrane receptor, vascular endothelial cell growth factor 165 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14786
  • Protein Name
    Neuropilin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Neuropilin 1/NRP1 using anti-Neuropilin 1/NRP1 antibody (A01324-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuropilin 1/NRP1 antigen affinity purified polyclonal antibody (Catalog # A01324-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuropilin 1/NRP1 at approximately 140 kDa. The expected band size for Neuropilin 1/NRP1 is at 103 kDa.
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