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IHC-P analysis of colon adenocarcinoma tissue using GTX83983 Neurotrophin 3 antibody [5C7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of colon adenocarcinoma tissue using GTX83983 Neurotrophin 3 antibody [5C7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of colon adenocarcinoma tissue using GTX83983 Neurotrophin 3 antibody [5C7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

Neurotrophin 3 antibody [5C7]

GTX83983
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNTF3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Neurotrophin 3 antibody [5C7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:40000. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C7
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4908
  • Target name
    NTF3
  • Target description
    neurotrophin 3
  • Target synonyms
    HDNF, NGF-2, NGF2, NT-3, NT3, neurotrophin-3, nerve growth factor 2, neurotrophic factor
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP20783
  • Protein Name
    Neurotrophin-3
  • Scientific Description
    Seems to promotes the survival of visceral and proprioceptive sensory neurons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Neurotrophin 3/NTF3 using anti-Neurotrophin 3/NTF3 antibody (A03348-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotrophin 3/NTF3 antigen affinity purified polyclonal antibody (Catalog # A03348-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotrophin 3/NTF3 at approximately 34KD. The expected band size for Neurotrophin 3/NTF3 is at 34KD.
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