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Whole cell extract (40 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Neurotrophin 4 antibody (GTX133792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (40 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Neurotrophin 4 antibody (GTX133792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (40 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Neurotrophin 4 antibody (GTX133792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Neurotrophin 4 antibody

GTX133792
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNTF4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Neurotrophin 4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.04 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4909
  • Target name
    NTF4
  • Target description
    neurotrophin 4
  • Target synonyms
    GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5, neurotrophin-4, neurotrophic factor 4, neurotrophic factor 5, neurotrophin 5 (neurotrophin 4/5), neurotrophin-5, neutrophic factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34130
  • Protein Name
    Neurotrophin-4
  • Scientific Description
    This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NT-4/NTF4 using anti-NT-4/NTF4 antibody (A06935-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT-4/NTF4 antigen affinity purified polyclonal antibody (Catalog # A06935-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT-4/NTF4 at approximately 24KD. The expected band size for NT-4/NTF4 is at 24KD.
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WB analysis of HEK293T cells transfected with Neurotrophin 4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83981 Neurotrophin 4 antibody [2E8]. Loading : 5 ug per lane
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