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ProductsBack/NTF4 / Neurotrophin 4 Antibody

NTF4 / Neurotrophin 4 Antibody

LS-C331246
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNTF4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NTF4 / Neurotrophin 4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 22kDa, while the observed MW by Western blot was 22kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:200), WB (1:500 - 1:2000) The predicted MW is 22kDa, while the observed MW by Western blot was 22kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4909
  • Target name
    NTF4
  • Target description
    neurotrophin 4
  • Target synonyms
    GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5, neurotrophin-4, neurotrophic factor 4, neurotrophic factor 5, neurotrophin 5 (neurotrophin 4/5), neurotrophin-5, neutrophic factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NT-4/NTF4 using anti-NT-4/NTF4 antibody (A06935-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT-4/NTF4 antigen affinity purified polyclonal antibody (Catalog # A06935-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NT-4/NTF4 at approximately 24KD. The expected band size for NT-4/NTF4 is at 24KD.
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