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ProductsBack/NF1A / NFIX Antibody

NF1A / NFIX Antibody

LS-C410923
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetNFIX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NF1A / NFIX Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 47kDa/48kDa/50kDa/54kDa/55kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 47kDa/48kDa/50kDa/54kDa/55kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4784
  • Target name
    NFIX
  • Target description
    nuclear factor I X
  • Target synonyms
    CTF, MALNS, MRSHSS, NF-I/X, NF1-X, NF1A, SOTOS2, nuclear factor 1 X-type, CCAAT-box-binding transcription factor, TGGCA-binding protein, nuclear factor 1/X
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFIX using anti-NFIX antibody (A04138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIX antigen affinity purified polyclonal antibody (Catalog # A04138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIX at approximately 55 kDa. The expected band size for NFIX is at 55 kDa.
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