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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA617941LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA617941LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA617941LA01HU at dilution of 1:100

NFIX Antibody

CSB-PA617941LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNFIX
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFIX Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4784
  • Target name
    NFIX
  • Target description
    nuclear factor I X
  • Target synonyms
    CTF, MALNS, MRSHSS, NF-I/X, NF1-X, NF1A, SOTOS2, nuclear factor 1 X-type, CCAAT-box-binding transcription factor, TGGCA-binding protein, nuclear factor 1/X
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14938
  • Protein Name
    Nuclear factor 1 X-type
  • Scientific Description
    Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFIX Polyclonal Antibody, Unconjugated (bs-11900R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
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Figure 1. Western blot analysis of NFIX using anti-NFIX antibody (A04138-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIX antigen affinity purified polyclonal antibody (Catalog # A04138-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIX at approximately 55 kDa. The expected band size for NFIX is at 55 kDa.
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