Bio-Connect
Western blot analysis of NFAT4 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of NFAT4 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of NFAT4 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

NFAT4 (phospho Ser165) antibody

GTX52339
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNFATC3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    NFAT4 (phospho Ser165) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB 1:500-1:2000, IF/ICC 1:100-1:500
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4775
  • Target name
    NFATC3
  • Target description
    nuclear factor of activated T cells 3
  • Target synonyms
    NFAT4; NF-AT4c; NF-ATc3; NFATX; nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12968
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 3
  • Scientific Description
    The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
TargetNFATC3
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetNFATC3
  • SizePrice
Product group Antibodies
Assay Genie
TargetNFATC3
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNFATC3
  • SizePrice
Western Blot analysis of HeLa cells using NFATc3 Polyclonal Antibody
Product group Antibodies
Cusabio
NFATC3 AntibodyCSB-PA003421
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNFATC3
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetNFATC3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
TargetNFATC3
  • SizePrice
Figure 1. Western blot analysis of NFAT4 using anti-NFAT4 antibody (A02727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4 antigen affinity purified polyclonal antibody (Catalog # A02727-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4 at approximately 115KD. The expected band size for NFAT4 is at 115KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNFATC3
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetNFATC3
  • SizePrice