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Western Blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.
Western Blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.
Western Blot analysis of HuvEc cells using NFATc1 Polyclonal Antibody.

NFATC1 Antibody

CSB-PA005617
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetNFATC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFATC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4772
  • Target name
    NFATC1
  • Target description
    nuclear factor of activated T cells 1
  • Target synonyms
    NF-ATC, NF-ATc1.2, NFAT2, NFATc, nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, nuclear factor of activated T-cells 'c', nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95644
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (A00340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody (Catalog # A00340-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD.
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