![IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:100 IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_IHC-P_13_w_23053121_725.webp)
IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:100
NFkB p65 antibody [GT1185]
GTX00947
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRELA
Overview
- SupplierGeneTex
- Product NameNFkB p65 antibody [GT1185]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1185
- ConjugateUnconjugated
- Gene ID5970
- Target nameRELA
- Target descriptionRELA proto-oncogene, NF-kB subunit
- Target synonymsAIF3BL3, CMCU, NFKB3, p65, transcription factor p65, NF-kappa-B p65delta3, NF-kappa-B transcription factor p65, nuclear factor NF-kappa-B p65 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A
- HostRabbit
- IsotypeIgG
- Protein IDQ04206
- Protein NameTranscription factor p65
- Scientific DescriptionNF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:100 IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_IHC-P_14_w_23053121_520.webp)
![WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Laoding : 25microg WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_1_w_23053121_277.webp)
![WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Loading : 25microg WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_2_w_23053121_405.webp)
![WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Loading : 25microg WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20200327_WB_3_w_23053121_334.webp)
![IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185]. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000 IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185]. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_20210323_IP_2_w_23053121_184.webp)
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00947/GTX00947_40000000132_20200306_WB_w_23053121_680.webp)



