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Western Blot analysis of COS7 cells using NFRkappaB Polyclonal Antibody
Western Blot analysis of COS7 cells using NFRkappaB Polyclonal Antibody
Western Blot analysis of COS7 cells using NFRkappaB Polyclonal Antibody

NFRKB Antibody

CSB-PA003423
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetNFRKB
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFRKB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4798
  • Target name
    NFRKB
  • Target description
    nuclear factor related to kappaB binding protein
  • Target synonyms
    INO80G, nuclear factor related to kappa-B-binding protein, DNA-binding protein R kappa B, INO80 complex subunit G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P4R8
  • Protein Name
    Nuclear factor related to kappa-B-binding protein
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFRKB using anti-NFRKB antibody (A06867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFRKB antigen affinity purified polyclonal antibody (Catalog # A06867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFRKB at approximately 175 kDa. The expected band size for NFRKB is at 139 kDa.
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