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NFRKB Antibody

LS-C496834
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNFRKB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NFRKB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 138kDa/139kDa/141kDa, while the observed MW by Western blot was 185kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 138kDa/139kDa/141kDa, while the observed MW by Western blot was 185kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4798
  • Target name
    NFRKB
  • Target description
    nuclear factor related to kappaB binding protein
  • Target synonyms
    INO80G, nuclear factor related to kappa-B-binding protein, DNA-binding protein R kappa B, INO80 complex subunit G
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFRKB using anti-NFRKB antibody (A06867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFRKB antigen affinity purified polyclonal antibody (Catalog # A06867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFRKB at approximately 175 kDa. The expected band size for NFRKB is at 139 kDa.
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