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Western blot All lanes: NFS1 antibody at 6.21microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 45 kDa Observed band size: 51 kDa
Western blot All lanes: NFS1 antibody at 6.21microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 45 kDa Observed band size: 51 kDa
Western blot All lanes: NFS1 antibody at 6.21microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 45 kDa Observed band size: 51 kDa

NFS1 Antibody

CSB-PA896542ESR1HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNFS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9054
  • Target name
    NFS1
  • Target description
    NFS1 cysteine desulfurase
  • Target synonyms
    COXPD52; cysteine desulfurase, mitochondrial; HUSSY-08; IscS; NFS1 nitrogen fixation 1 homolog; NIFS; nitrogen fixation 1 (S. cerevisiae, homolog); nitrogen-fixing bacteria S-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y697
  • Protein Name
    Cysteine desulfurase, mitochondrial
  • Scientific Description
    Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFS1 using anti-NFS1 antibody (A05061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse B16-F10 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFS1 antigen affinity purified polyclonal antibody (Catalog # A05061-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFS1 at approximately 45 kDa. The expected band size for NFS1 is at 50 kDa.
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