Western Blot of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 microg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4oC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: doublet of ~ 42 kDa for NF-Y A.
Western Blot of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 microg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4oC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: doublet of ~ 42 kDa for NF-Y A.
NFYA antibody
GTX26558
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, Other Application
Product group Antibodies
ReactivityHuman, Monkey
TargetNFYA
Overview
- SupplierGeneTex
- Product NameNFYA antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.6 mg/ml
- ConjugateUnconjugated
- Gene ID4800
- Target nameNFYA
- Target descriptionnuclear transcription factor Y subunit alpha
- Target synonymsCAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit
- HostRabbit
- IsotypeIgG
- Protein IDP23511
- Protein NameNuclear transcription factor Y subunit alpha
- Scientific DescriptionThe Y box is a CCAAT box that is bound by the heteromeric DNA binding protein, NF-Y (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1, which also bind CCAAT-like sequences, NF-Y exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30% of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A, B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.
- ReactivityHuman, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203