NFYA antibody

GeneTex

NFYA antibody

9

WB: 1:1000. ELISA: 1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, Other Application

Research Use Only

Polyclonal

0.6 mg/ml

Unconjugated

NFYA

nuclear transcription factor Y subunit alpha

CAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit

Rabbit

IgG

Nuclear transcription factor Y subunit alpha

The Y box is a CCAAT box that is bound by the heteromeric DNA binding protein, NF-Y (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1, which also bind CCAAT-like sequences, NF-Y exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30% of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A, B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.

-20°C or -80°C,2°C to 8°C

12352203