GeneTexs anti-NF-Y (A Subunit) antibody was diluted at 10 microg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
![CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 microg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4oC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48667/GTX48667_20160330_WB_w_23060823_896.webp "CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 microg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4oC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.")
GeneTexs anti-NF-Y (A Subunit) antibody was diluted at 10 microg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
NFYA antibody
GTX48667
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetNFYA
Overview
- SupplierGeneTex
- Product NameNFYA antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000-1:10000. IHC-P: 1:400. ELISA: 1:20000-1:100000. EMSA: 2 microL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID4800
- Target nameNFYA
- Target descriptionnuclear transcription factor Y subunit alpha
- Target synonymsCAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit
- HostRabbit
- IsotypeIgG
- Protein IDP23511
- Protein NameNuclear transcription factor Y subunit alpha
- Scientific DescriptionNuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203