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Western Blot analysis of Jurkat cells using NGF Polyclonal Antibody
Western Blot analysis of Jurkat cells using NGF Polyclonal Antibody
Western Blot analysis of Jurkat cells using NGF Polyclonal Antibody

NGF Antibody

CSB-PA003446
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNGF
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Overview

  • Supplier
    Cusabio
  • Product Name
    NGF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4803
  • Target name
    NGF
  • Target description
    nerve growth factor
  • Target synonyms
    Beta-NGF, HSAN5, NGFB, beta-nerve growth factor, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit, pro-nerve growth factor long
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01138
  • Protein Name
    Beta-nerve growth factor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NGF using anti-NGF antibody (A00341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27KD. The expected band size for NGF is at 27KD.
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