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Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

NGFB Polyclonal Antibody

BS-0067R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep
TargetNGF
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Overview

  • Supplier
    Bioss
  • Product Name
    NGFB Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4803
  • Target name
    NGF
  • Target description
    nerve growth factor
  • Target synonyms
    Beta-NGF, HSAN5, NGFB, beta-nerve growth factor, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit, pro-nerve growth factor long
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01138
  • Protein Name
    Beta-nerve growth factor
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • The Combination of Adipose-derived Schwann-like Cells and Acellular Nerve Allografts Promotes Sciatic Nerve Regeneration and Repair through the JAK2/STAT3 Signaling Pathway in Rats. Fu XM et al., 2019 Dec 1, Neuroscience
    Read this paper
  • Connexin 43 is involved in the sympathetic atrial fibrillation in canine and canine atrial myocytes. Shu C et al., 2017 Jul, Anatol J Cardiol
    Read this paper
  • Choline supplementation alleviates fluoride-induced testicular toxicity by restoring the NGF and MEK expression in mice. Zhang J et al., 2016 Nov 1, Toxicol Appl Pharmacol
    Read this paper
  • Effect of Low-level Vagus Nerve Stimulation on Cardiac Remodeling in a Rapid Atrial Pacing-induced Canine Model of Atrial Fibrillation. Lu Y et al., 2016 Mar, J Cardiovasc Pharmacol
    Read this paper
  • Biological and clinical significance of p75NTR expression in laryngeal squamous epithelia and laryngocarcinoma. Li X et al., 2012 Mar, Acta Otolaryngol
    Read this paper
  • Therapeutic potential of in utero mesenchymal stem cell (MSCs) transplantation in rat foetuses with spina bifida aperta. Li H et al., 2012 Jul, J Cell Mol Med
    Read this paper

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Figure 1. Western blot analysis of NGF using anti-NGF antibody (A00341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27KD. The expected band size for NGF is at 27KD.
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