![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84041 NIT1 antibody [3A11]. Loading : 10 ug per lane Dilution : 1:500 WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84041 NIT1 antibody [3A11]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_4440_WB_w_23061420_840.webp)
WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84041 NIT1 antibody [3A11]. Loading : 10 ug per lane Dilution : 1:500
NIT1 antibody [3A11]
GTX84041
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNIT1
Overview
- SupplierGeneTex
- Product NameNIT1 antibody [3A11]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A11
- Concentration0.87 mg/ml
- ConjugateUnconjugated
- Gene ID4817
- Target nameNIT1
- Target descriptionnitrilase 1
- Target synonymsBSVD4, deaminated glutathione amidase, dGSH amidase
- HostMouse
- IsotypeIgG1
- Protein IDQ86X76
- Protein NameDeaminated glutathione amidase
- Scientific DescriptionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells - this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![ICC/IF analysis of HT29 cells using GTX84041 NIT1 antibody [3A11]. ICC/IF analysis of HT29 cells using GTX84041 NIT1 antibody [3A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_924_ICCIF_w_23061420_122.webp)
![FACS analysis of HEK293T cells transfected with either NIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84041 NIT1 antibody [3A11]. FACS analysis of HEK293T cells transfected with either NIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84041 NIT1 antibody [3A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_283_FACS_w_23061420_829.webp)
![IHC-P analysis of pancreas tissue using GTX84041 NIT1 antibody [3A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of pancreas tissue using GTX84041 NIT1 antibody [3A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_2228_IHC-P_w_23061420_517.webp)
![ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84041 NIT1 antibody [3A11]. ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84041 NIT1 antibody [3A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_923_ICCIF_w_23061420_422.webp)
![WB analysis of HEK293T cells transfected with NIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84041 NIT1 antibody [3A11]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with NIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84041 NIT1 antibody [3A11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_4087_WB_w_23061420_839.webp)
![IHC-P analysis of kidney tissue using GTX84041 NIT1 antibody [3A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50 IHC-P analysis of kidney tissue using GTX84041 NIT1 antibody [3A11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84041/GTX84041_2227_IHC-P_w_23061420_357.webp)





![ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84042 NIT1 antibody [4D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84042/GTX84042_925_ICCIF_w_23061420_462.webp)
