IHC-P analysis of human ovary adenocarcinoma tissue using GTX84042 NIT1 antibody [4D12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![FACS analysis of HEK293T cells transfected with either NIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84042 NIT1 antibody [4D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84042/GTX84042_284_FACS_w_23061420_256.webp "FACS analysis of HEK293T cells transfected with either NIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84042 NIT1 antibody [4D12].")
![WB analysis of HEK293T cells transfected with NIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84042 NIT1 antibody [4D12]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84042/GTX84042_4089_WB_w_23061420_449.webp "WB analysis of HEK293T cells transfected with NIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84042 NIT1 antibody [4D12]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84042 NIT1 antibody [4D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84042/GTX84042_925_ICCIF_w_23061420_462.webp "ICC/IF analysis of COS7 cells transiently transfected with NIT1 plasmid using GTX84042 NIT1 antibody [4D12].")
![ICC/IF analysis of HT29 cells using GTX84042 NIT1 antibody [4D12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84042/GTX84042_926_ICCIF_w_23061420_124.webp "ICC/IF analysis of HT29 cells using GTX84042 NIT1 antibody [4D12].")
IHC-P analysis of human ovary adenocarcinoma tissue using GTX84042 NIT1 antibody [4D12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
NIT1 antibody [4D12]
GTX84042
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNIT1
Overview
- SupplierGeneTex
- Product NameNIT1 antibody [4D12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D12
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID4817
- Target nameNIT1
- Target descriptionnitrilase 1
- Target synonymsdeaminated glutathione amidase; dGSH amidase
- HostMouse
- IsotypeIgG1
- Protein IDQ86X76
- Protein NameDeaminated glutathione amidase
- Scientific DescriptionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells - this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203