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NIX / BNIP3L Antibody

LS-C402988
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBNIP3L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NIX / BNIP3L Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID665
  • Target name
    BNIP3L
  • Target description
    BCL2 interacting protein 3 like
  • Target synonyms
    BNIP3a, NIP3L, NIX, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BCL2/adenovirus E1B 19-kd protein-interacting protein 3a, BCL2/adenovirus E1B 19kDa interacting protein 3 like, NIP-3-like protein X, NIP3-like protein X, adenovirus E1B19k-binding protein B5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BNIP3L using anti-BNIP3L antibody (A03107-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3L antigen affinity purified polyclonal antibody (Catalog # A03107-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BNIP3L at approximately 35 kDa. The expected band size for BNIP3L is at 24 kDa.
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