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NKp46 antibody

GTX05037
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNCR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NKp46 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200-1:1000. IHC-P: 1:25-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9437
  • Target name
    NCR1
  • Target description
    natural cytotoxicity triggering receptor 1
  • Target synonyms
    CD335, LY94, NK-p46, NKP46, natural cytotoxicity triggering receptor 1, NK cell-activating receptor, lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46), natural killer cell p46-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76036
  • Protein Name
    Natural cytotoxicity triggering receptor 1
  • Scientific Description
    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCR1 using anti-NCR1 antibody (A01516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR1 antigen affinity purified polyclonal antibody (Catalog # A01516-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCR1 at approximately 36KD. The expected band size for NCR1 is at 34KD.
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