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NLRC5 Antibody

CAB16710
Assay Genie
TargetCCL4
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NLRC5 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6351
  • Target name
    CCL4
  • Target description
    C-C motif chemokine ligand 4
  • Target synonyms
    ACT2; AT744.1; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; G-26; G-26 T-lymphocyte-secreted protein; HC21; LAG1; LAG-1; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); T-cell activation protein 2
  • Host
    Rabbit
  • Protein IDP13236
  • Protein Name
    C-C motif chemokine 4
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL4 using anti-CCL4 antibody (PB9572). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL4 antigen affinity purified polyclonal antibody (Catalog # PB9572) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL4 at approximately 11 kDa. The expected band size for CCL4 is at 11 kDa.
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