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Western Blot analysis of HeLa cells using NM23-H1 Polyclonal Antibody
Western Blot analysis of HeLa cells using NM23-H1 Polyclonal Antibody
Western Blot analysis of HeLa cells using NM23-H1 Polyclonal Antibody

NME1 Antibody

CSB-PA003460
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNME1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NME1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4830
  • Target name
    NME1
  • Target description
    NME/NM23 nucleoside diphosphate kinase 1
  • Target synonyms
    AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1, nucleoside diphosphate kinase A, NDP kinase A, epididymis secretory sperm binding protein, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1, protein (NM23A) expressed in, tumor metastatic process-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15531
  • Protein Name
    Nucleoside diphosphate kinase A
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.
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