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ProductsBack/Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Polyclonal Antibody

Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Polyclonal Antibody

CAU21647
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetNME1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4830
  • Target name
    NME1
  • Target description
    NME/NM23 nucleoside diphosphate kinase 1
  • Target synonyms
    AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1, nucleoside diphosphate kinase A, NDP kinase A, epididymis secretory sperm binding protein, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1, protein (NM23A) expressed in, tumor metastatic process-associated protein
  • Host
    Rabbit
  • Protein IDP15531
  • Protein Name
    Nucleoside diphosphate kinase A
  • Scientific Description
    The Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.
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