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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Nos-1 Polyclonal Antibody, Unconjugated (bs-0156R) at 1:400followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Nos-1 Polyclonal Antibody, Unconjugated (bs-0156R) at 1:400followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Nos-1 Polyclonal Antibody, Unconjugated (bs-0156R) at 1:400followed by conjugation to the secondary antibody and DAB staining.

nNos Polyclonal Antibody

BS-0156R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNOS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    nNos Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4842
  • Target name
    NOS1
  • Target description
    nitric oxide synthase 1
  • Target synonyms
    bNOS; constitutive NOS; IHPS1; NC-NOS; neuronal NOS; nitric oxide synthase 1 (neuronal); nitric oxide synthase, brain; nNOS; N-NOS; NOS; NOS type I; peptidyl-cysteine S-nitrosylase NOS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29475
  • Protein Name
    Nitric oxide synthase, brain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Erythropoietin promotes retinal angiogenesis in a mouse model. Wen CT et al., 2014 Dec, Mol Med Rep
    Read more
  • Neurochemical plasticity of nitric oxide synthase isoforms in neurogenic detrusor overactivity after spinal cord injury. Zhang F et al., 2011 Oct, Neurochem Res
    Read more
  • The diversity of nNOS gene expression in avulsion-injured spinal motoneurons among laboratory rodents. Wang J et al., 2010 Jan 1, Nitric Oxide
    Read more

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Figure 1. Western blot analysis of nNOS (neuronal)/NOS1 using anti-nNOS (neuronal)/NOS1 antibody (A01070-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS (neuronal)/NOS1 antigen affinity purified polyclonal antibody (Catalog # A01070-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nNOS (neuronal)/NOS1 at approximately 161 kDa. The expected band size for nNOS (neuronal)/NOS1 is at 161 kDa.
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