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IHC-P analysis of human ovary tissue using GTX84023 Noggin antibody [4C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human ovary tissue using GTX84023 Noggin antibody [4C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human ovary tissue using GTX84023 Noggin antibody [4C12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

Noggin antibody [4C12]

GTX84023
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNOG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Noggin antibody [4C12] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9241
  • Target name
    NOG
  • Target description
    noggin
  • Target synonyms
    SYM1, SYNS1, SYNS1A, noggin, symphalangism 1 (proximal)
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13253
  • Protein Name
    Noggin
  • Scientific Description
    Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Noggin using anti-Noggin antibody (A04448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A375 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Noggin antigen affinity purified polyclonal antibody (Catalog # A04448-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Noggin at approximately 26KD. The expected band size for Noggin is at 26KD.
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