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Western Blot analysis of Hela cell using NOL9 Polyclonal Antibody at dilution of 1:1250
Western Blot analysis of Hela cell using NOL9 Polyclonal Antibody at dilution of 1:1250
Western Blot analysis of Hela cell using NOL9 Polyclonal Antibody at dilution of 1:1250

NOL9 Polyclonal Antibody

E-AB-14721
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetNOL9
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Overview

  • Supplier
    Elabscience
  • Product Name
    NOL9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79707
  • Target name
    NOL9
  • Target description
    nucleolar protein 9
  • Target synonyms
    Grc3, NET6, polynucleotide 5'-hydroxyl-kinase NOL9, polynucleotide 5'-kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5SY16
  • Protein Name
    Polynucleotide 5'-hydroxyl-kinase NOL9
  • Scientific Description
    Nucleolar protein 9is aproteinthat in humans is encoded by theNOL9gene. Polynucleotide 5-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5-kinase activities. Probably binds RNA.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOL9 using anti-NOL9 antibody (A11105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL9 antigen affinity purified polyclonal antibody (A11105-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL9 at approximately 79 kDa. The expected band size for NOL9 is at 79 kDa.
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