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Confocal immunofluorescence analysis of U2OS cells using NOP58 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using NOP58 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using NOP58 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

NOP58 Polyclonal Antibody

E-AB-64937
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NOP58 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOP58 using anti-NOP58 antibody (A05070-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP58 antigen affinity purified polyclonal antibody (Catalog # A05070-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP58 at approximately 70 kDa. The expected band size for NOP58 is at 60 kDa.
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