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Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining

iNOS Polyclonal Antibody

BS-2072R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNos2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NOS-2/iNOS Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID18126
  • Target name
    Nos2
  • Target description
    nitric oxide synthase 2, inducible
  • Target synonyms
    inducible nitric oxide synthase; inducible NO synthase; inducible NOS; iNOS; i-NOS; MAC-NOS; macrophage NOS; N; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase, inducible; No; NOS type II; Nos-2; Nos2a; NOS-II; peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29477
  • Protein Name
    Nitric oxide synthase, inducible
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Wedn AM et al., 2020 Jan, Saudi Pharm J
    Read more
  • The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. Wu TH et al., 2019, J Oncol
    Read more
  • Thymoquinone-PLGA-PVA Nanoparticles Ameliorate Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulation of Inflammatory Cytokines and iNOS Signaling. Saghir SAM et al., 2019 Nov 11, Animals (Basel)
    Read more
  • The Activation of Heat-Shock Protein After Copper(II) and/or Arsenic(III)-Induced Imbalance of Homeostasis, Inflammatory Response in Chicken Rectum. Yang X et al., 2020 Jun, Biol Trace Elem Res
    Read more
  • Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice. Vairappan B et al., 2019 Jul, Mol Neurobiol
    Read more
  • Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells. Yang B et al., 2018 Feb 6, Anal Chem
    Read more
  • Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken. Shao Y et al., 2018 Mar, Environ Sci Pollut Res Int
    Read more
  • Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis. Liu Q et al., 2017 Dec, Biomed Pharmacother
    Read more
  • Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Sun G et al., 2017 Oct 30, Exp Anim
    Read more
  • The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection. Zhao M et al., 2017 Jun 8, Int J Mol Sci
    Read more

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Figure 1. Western blot analysis of iNOS/Nos2 using anti-iNOS/Nos2 antibody (A00368-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-iNOS/Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for iNOS/Nos2 at approximately 130 kDa. The expected band size for iNOS/Nos2 is at 130 kDa.
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