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Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining

iNOS Polyclonal Antibody

BS-2072R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNos2
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Overview

  • Supplier
    Bioss
  • Product Name
    iNOS Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID18126
  • Target name
    Nos2
  • Target description
    nitric oxide synthase 2, inducible
  • Target synonyms
    MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS, nitric oxide synthase, inducible, NOS type II, inducible NO synthase, inducible NOS, inducible nitric oxide synthase, macrophage NOS, nitric oxide synthase 2, inducible, macrophage, peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29477
  • Protein Name
    Nitric oxide synthase, inducible
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wedn AM, El-Gowilly SM, El-Mas MM. Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Saudi Pharm J. 2020,28(1):127-135. doi: 10.1016/j.jsps.2019.11.014
    Read this paper
  • Wu TH, Yeh KY, Wang CH, et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019,2019:9206951. doi: 10.1155/2019/9206951
    Read this paper
  • Saghir SAM, Al-Gabri NA, Khafaga AF, et al. Thymoquinone-PLGA-PVA Nanoparticles Ameliorate Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulation of Inflammatory Cytokines and iNOS Signaling. Animals (Basel). 2019,9(11). doi: 10.3390/ani9110951
    Read this paper
  • Yang X, Zhao H, Wang Y, et al. The Activation of Heat-Shock Protein After Copper(II) and/or Arsenic(III)-Induced Imbalance of Homeostasis, Inflammatory Response in Chicken Rectum. Biol Trace Elem Res. 2020,195(2):613-623. doi: 10.1007/s12011-019-01871-8
    Read this paper
  • Vairappan B, Sundhar M, Srinivas BH. Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl(4)-Induced Cirrhotic Mice. Mol Neurobiol. 2019,56(7):4718-4729. doi: 10.1007/s12035-018-1389-x
    Read this paper
  • Yang B, Chen B, He M, et al. Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells. Anal Chem. 2018,90(3):2355-2361. doi: 10.1021/acs.analchem.7b04927
    Read this paper
  • Shao Y, Zhao H, Wang Y, et al. Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken. Environ Sci Pollut Res Int. 2018,25(8):7719-7729. doi: 10.1007/s11356-017-1042-7
    Read this paper
  • Liu Q, Li H, Yang J, et al. Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis. Biomed Pharmacother. 2017,96:1363-1370. doi: 10.1016/j.biopha.2017.11.066
    Read this paper
  • Sun G, Yang W, Zhang Y, et al. Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Exp Anim. 2017,66(4):303-312. doi: 10.1538/expanim.16-0102
    Read this paper
  • Zhao M, Jiang Q, Wang W, et al. The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection. Int J Mol Sci. 2017,18(6). doi: 10.3390/ijms18061229
    Read this paper

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Figure 1. Western blot analysis of iNOS/Nos2 using anti-iNOS/Nos2 antibody (A00368-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-iNOS/Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for iNOS/Nos2 at approximately 130 kDa. The expected band size for iNOS/Nos2 is at 130 kDa.
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