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Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody (GTX127952) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

iNOS antibody

GTX127952
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetNos2
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Overview

  • Supplier
    GeneTex
  • Product Name
    iNOS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18126
  • Target name
    Nos2
  • Target description
    nitric oxide synthase 2, inducible
  • Target synonyms
    MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS, nitric oxide synthase, inducible, NOS type II, inducible NO synthase, inducible NOS, inducible nitric oxide synthase, macrophage NOS, nitric oxide synthase 2, inducible, macrophage, peptidyl-cysteine S-nitrosylase NOS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29477
  • Protein Name
    Nitric oxide synthase, inducible
  • Scientific Description
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of iNOS/Nos2 using anti-iNOS/Nos2 antibody (A00368-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-iNOS/Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for iNOS/Nos2 at approximately 130 kDa. The expected band size for iNOS/Nos2 is at 130 kDa.
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