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ProductsBack/NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI4C9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOTCH1 (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOTCH1. Positive lysates LY402602 (100ug) and LC402602 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOTCH1 (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOTCH1. Positive lysates LY402602 (100ug) and LC402602 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOTCH1 (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOTCH1. Positive lysates LY402602 (100ug) and LC402602 (20ug) can be purchased separately from OriGene.

NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI4C9]

CF500248
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOTCH1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI4C9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4C9
  • Gene ID4851
  • Target name
    NOTCH1
  • Target description
    notch receptor 1
  • Target synonyms
    AOS5, AOVD1, TAN1, hN1, neurogenic locus notch homolog protein 1, Notch homolog 1, translocation-associated, notch 1, translocation-associated notch protein TAN-1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP46531
  • Protein Name
    Neurogenic locus notch homolog protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) Notch1 mouse monoclonal antibody, clone OTI4C9 (formerly 4C9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Notch1 using anti-Notch1 antibody (A00033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Notch1 antigen affinity purified polyclonal antibody (Catalog # A00033-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Notch1 at approximately 272KD. The expected band size for Notch1 is at 272KD.
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