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Anti-Notch 1 [E6]

Ab00175-23.0
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNOTCH1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Notch 1 [E6]
  • Delivery Days Customer
    7
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E6
  • Gene ID4851
  • Target name
    NOTCH1
  • Target description
    notch receptor 1
  • Target synonyms
    AOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46531
  • Protein Name
    Neurogenic locus notch homolog protein 1
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original human scFv format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, Mouse
  • Reactivity Supplier Note
    This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Notch1 using anti-Notch1 antibody (A00033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Notch1 antigen affinity purified polyclonal antibody (Catalog # A00033-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Notch1 at approximately 272KD. The expected band size for Notch1 is at 272KD.
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