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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015950LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015950LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015950LA01HU at dilution of 1:100

NOTCH2 Antibody

CSB-PA015950LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOTCH2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOTCH2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4853
  • Target name
    NOTCH2
  • Target description
    notch receptor 2
  • Target synonyms
    AGS2; HJCYS; hN2; neurogenic locus notch homolog protein 2; notch 2; Notch homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04721
  • Protein Name
    Neurogenic locus notch homolog protein 2
  • Scientific Description
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH2 antigen affinity purified polyclonal antibody (Catalog # A00518-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOTCH2 at approximately 110 kDa, 300 kDa. The expected band size for NOTCH2 is at 265 kDa.
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