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NOTCH2 Antibody

LS-C408224
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNOTCH2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NOTCH2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 265kDa, while the observed MW by Western blot was 120kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:200), WB (1:500 - 1:2000) The predicted MW is 265kDa, while the observed MW by Western blot was 120kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4853
  • Target name
    NOTCH2
  • Target description
    notch receptor 2
  • Target synonyms
    AGS2, HJCYS, hN2, neurogenic locus notch homolog protein 2, Notch homolog 2, notch 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH2 antigen affinity purified polyclonal antibody (Catalog # A00518-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOTCH2 at approximately 110 kDa, 300 kDa. The expected band size for NOTCH2 is at 265 kDa.
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