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ProductsBack/NOX4 antibody [N3C3]

NOX4 antibody [N3C3]

GTX121929
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOX4
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Overview

  • Supplier
    GeneTex
  • Product Name
    NOX4 antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.05 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50507
  • Target name
    NOX4
  • Target description
    NADPH oxidase 4
  • Target synonyms
    KOX, KOX-1, RENOX, NADPH oxidase 4, kidney oxidase-1, kidney superoxide-producing NADPH oxidase, renal NAD(P)H-oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPH5
  • Protein Name
    NADPH oxidase 4
  • Scientific Description
    This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shen CJ, Chan RH, Lin BW, et al. Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation. Theranostics. 2023,13(13):4650-4666. doi: 10.7150/thno.85855
    Read this paper
  • Su H, Yang Z, Zhang Y, et al. Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction. iScience. 2023,26(4):106338. doi: 10.1016/j.isci.2023.106338
    Read this paper
  • Chiou WC, Huang GJ, Chang TY, et al. Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway. Biomed Pharmacother. 2023,161:114481. doi: 10.1016/j.biopha.2023.114481
    Read this paper
  • Chen KC, Chen CR, Chen CY, et al. Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics. J Clin Med. 2021,11(1). doi: 10.3390/jcm11010135
    Read this paper
  • Tsai HY, Hsu YJ, Lu CY, et al. Pharmacological Activation Of Aldehyde Dehydrogenase 2 Protects Against Heatstroke-Induced Acute Lung Injury by Modulating Oxidative Stress and Endothelial Dysfunction. Front Immunol. 2021,12:740562. doi: 10.3389/fimmu.2021.740562
    Read this paper
  • Pan MS, Wang H, Ansari KH, et al. Gallbladder cancer-associated fibroblasts promote vasculogenic mimicry formation and tumor growth in gallbladder cancer via upregulating the expression of NOX4, a poor prognosis factor, through IL-6-JAK-STAT3 signal pathway. J Exp Clin Cancer Res. 2020,39(1):234. doi: 10.1186/s13046-020-01742-4
    Read this paper
  • Cheng D, Chen L, Tu W, et al. Protective effects of valsartan administration on doxorubicin‑induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling. Mol Med Rep. 2020,22(5):4151-4162. doi: 10.3892/mmr.2020.11521
    Read this paper
  • Miyano K, Okamoto S, Yamauchi A, et al. The NADPH oxidase NOX4 promotes the directed migration of endothelial cells by stabilizing vascular endothelial growth factor receptor 2 protein. J Biol Chem. 2020,295(33):11877-11890. doi: 10.1074/jbc.RA120.014723
    Read this paper
  • Yu S, Zhao G, Han F, et al. Muscone relieves inflammatory pain by inhibiting microglial activation-mediated inflammatory response via abrogation of the NOX4/JAK2-STAT3 pathway and NLRP3 inflammasome. Int Immunopharmacol. 2020,82:106355. doi: 10.1016/j.intimp.2020.106355
    Read this paper
  • Chen YH, Lu HI, Lo CM, et al. NOX4 overexpression is a poor prognostic factor in patients undergoing curative esophagectomy for esophageal squamous cell carcinoma. Surgery. 2020,167(3):620-627. doi: 10.1016/j.surg.2019.11.017
    Read this paper

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293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NADPH oxidase 4 Antibody(bs-4730R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of NOX4 using anti-NOX4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human T47D whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.
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