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ProductsBack/NOX4 Antibody (Preservative Free)

NOX4 Antibody (Preservative Free)

LS-C815389
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNOX4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NOX4 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID50507
  • Target name
    NOX4
  • Target description
    NADPH oxidase 4
  • Target synonyms
    kidney oxidase-1; kidney superoxide-producing NADPH oxidase; KOX; KOX-1; NADPH oxidase 4; renal NAD(P)H-oxidase; RENOX
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOX4 using anti-NOX4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human T47D whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.
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